Cancer Cell Fitness Is Dynamic

Abstract Several phenotypes that impact the capacity of cancer cells to survive and proliferate are dynamic. Here we used number in colonies as an assessment fitness devised a novel method called Dynamic Fitness Analysis (DynaFit) measure dynamics over course colony formation. DynaFit is based on variance growth rate population founder compared with different sizes. revealed cell lines, primary cells, fibroblasts under unhindered conditions Key cellular mechanisms such ERK signaling cell-cycle synchronization differed significantly among after 2 4 generations became indistinguishable from randomly sampled regarding these features. In presence cytotoxic agents, reduced their when cell, indicating dynamic nature drug. This finding was supported by measurable differences DNA damage induction senescence colonies. The epigenetic modulators during formation stabilized for at least four generations. Collectively, results support understanding its modulation fundamental aspect be considered comprehending biology response therapeutic interventions. Significance: Cancer behavior mediated asymmetric mitosis, activity, duration, repair absence or